Labdisk Integrated Dna Extraction from Whole Blood Using Magnetic Particles
نویسندگان
چکیده
Integration of sample preparation is a key issue in the development of point of care diagnostic devices. We address the integration of a magnetic-particle based DNA extraction from 200 μl of whole blood into a centrifugal-microfluidic LabDisk. Quality of LabDisk extracted DNA was compared to DNA extracted by commercial spin columns as golden standard with four independent extractions from one blood sample. Experiments reveal comparable recovery of human growth factor gene determined by qPCR (c1:10 LabDisk = 4.6 +/0.7 ng/μl vs. c1:10 spin-column = 4.1 +/0.4 ng/μl), comparable purity (A260/A280 ~ 1,8 +/0.1), and slightly increased ethanol concentrations in LabDisk extracted DNA 5.9 +/2.4 % compared to 3.3 +/0.2 % in case of spin-columns.
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